1. A mutation that disrupts the function of spliceosome components would primarily affect ______________ in a eukaryotic cell

 DNA replication

 Post-transcriptional mRNA processing

 Chromatin remodeling

 Transcription initiation

2. Which RNA polymerase is responsible for synthesizing pre-mRNA (messenger RNA) in eukaryotes?

 RNA polymerase III

 RNA polymerase I

 Primase

 RNA polymerase II

3. A mutation that disrupts the function of the TATA-binding protein (TBP) would most directly affect _______________________ in a eukaryotic cell?

 Formation of the transcription initiation complex

 Translation elongation

 RNA splicing

 Protein folding

4. A transcription factor that recruits histone deacetylase (HDAC) will _______

 Activate transcription

 Promote gene repression via chromatin condensation

 Enhance RNA stability

 Increase chromatin accessibility

5. During cellular differentiation, two cells have identical DNA but different phenotypes. This is primarily due to _______

 Protein degradation errors

 Differential gene expression regulated epigenetically

 Chromosomal rearrangement

 Gene mutation

6. A eukaryotic gene shows high transcription but low protein levels. The most plausible explanation is _______

 Post-transcriptional regulation

 Lack of enhancer elements

 DNA methylation in the coding region

 Inactive promoter

7. If histone H3 lysine 9 (H3K9) is extensively trimethylated across a chromosomal region in eukaryotic cells, what is the most likely functional consequence?

 Increased homologous recombination frequency

 Active transcription due to chromatin relaxation

 Enhanced efficiency of mRNA translation

 Formation of transcriptionally silent heterochromatin

8. In eukaryotes, enzyme ____________ synthesizes short RNA primers during DNA replication (not transcription)

 Primase

 RNA polymerase III

 RNA polymerase II

 Ligase

9. Which RNA polymerase in eukaryotes is primarily responsible for synthesizing major ribosomal RNA (rRNA) precursors (except 5S rRNA)?

 Primase

 RNA polymerase I

 RNA polymerase III

 RNA polymerase II

10. In eukaryotes, a gene’s promoter contains binding sites for three transcription factors, and transcription occurs only when all three are bound simultaneously. This regulatory strategy is known as _______

 Gene silencing that prevents transcription

 Combinatorial control, where specific combinations of factors determine expression

 Operon repression as in prokaryotic systems

 Attenuation leading to premature termination

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Question 1 of 10

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Gene Expression MCQs

Gene Expression is the process by which information encoded in DNA is converted into functional products—primarily proteins or functional RNAs.

It occurs in two main stages:

• Transcription: DNA is copied into messenger RNA (mRNA).
• Translation: mRNA is decoded by ribosomes to synthesize proteins.

Gene expression is tightly regulated at multiple levels (epigenetic, transcriptional, post-transcriptional, and translational), allowing cells to control when, where, and how much of a gene product is produced, which underlies cell differentiation, development, and environmental responses.